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Metabolism of valine by the filamentous fungus Arthrobotrys conoides.
- Source :
-
Journal of bacteriology [J Bacteriol] 1970 Jul; Vol. 103 (1), pp. 131-9. - Publication Year :
- 1970
-
Abstract
- Uptake of valine by Arthrobotrys conoides was an active process and was independent of its incorporation into cellular protein. Chemical fractionation of cells supplied with (14)C-l-valine for different time intervals revealed that the amino acid initially entered a pool of metabolic intermediates and was extractable with cold trichloroacetic acid. After a 4-min interval, some intracellular valine was incorporated into cell proteins, but most underwent metabolic transformation to a variety of products that included carboxylic acids and other amino acids. Carbon derived from valine was not localized in the lipid or nucleic acid fraction of cells, but some was completely oxidized and recovered as metabolic (14)CO(2). Autoradiograms of paper and thin-layer chromatograms of acid hydrolysates of cellular protein identified the following amino acids as having originated from valine: glutamate, aspartate, alanine, and leucine. Similar analysis of cold trichloroacetic acid extracts established that (14)C supplied as l-valine had been transformed also to alpha-ketoisovalerate, isobutyrate, propionate, succinate, malate, oxalacetate, pyruvate, and alpha-ketoglutarate. Pathways for transformation of the carbon skeleton of valine to various metabolic products are proposed.
- Subjects :
- Alanine biosynthesis
Amino Acids metabolism
Aspartic Acid biosynthesis
Autoradiography
Carbon Dioxide biosynthesis
Carbon Isotopes
Chromatography, Paper
Chromatography, Thin Layer
Dinitrophenols
Glutamates biosynthesis
Hydrazines
Keto Acids biosynthesis
Leucine biosynthesis
Mitosporic Fungi growth & development
Spores growth & development
Time Factors
Trichloroacetic Acid
Mitosporic Fungi metabolism
Spores metabolism
Valine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 103
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 5463679
- Full Text :
- https://doi.org/10.1128/jb.103.1.131-139.1970