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Immunogold for detection of antigen on nitrocellulose paper.
- Source :
-
Analytical biochemistry [Anal Biochem] 1984 Oct; Vol. 142 (1), pp. 221-5. - Publication Year :
- 1984
-
Abstract
- The application of immunogold for the detection of antigen-antibody complexes on nitrocellulose paper is described. Tobacco mosaic virus (TMV) protein, either purified or in leaf extract, was bound to the nitrocellulose paper and then exposed to rabbit anti-TMV serum. The antigen-antibody complexes were detected by gold-labeled goat anti-rabbit immunoglobulin G. The gold-labeled antibody is directly visible because of its pink color. This method can detect 1-5 pg of TMV protein, either in purified form or in the unpurified plant extract, with high specificity.
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 142
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6083735
- Full Text :
- https://doi.org/10.1016/0003-2697(84)90542-6