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Leukotriene B formation by neutrophils from essential fatty acid-deficient rats.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1984 Oct 10; Vol. 259 (19), pp. 11784-9. - Publication Year :
- 1984
-
Abstract
- Analysis of neutrophil phospholipids from rats fed an essential fatty acid-deficient diet revealed a 33% reduction in arachidonate and a 90% reduction in linoleate compared to neutrophil phospholipids of rats fed a normal diet. The neutrophil phospholipids from rats fed the essential fatty acid-deficient diet also contained significant amounts of 5,8,11-eicosatrienoate, a fatty acid not found in the neutrophils of rats fed a normal diet. Analysis of the production of leukotrienes of the B series by ionophore-stimulated neutrophils from rats fed an essential fatty acid-deficient diet revealed a 87% reduction in leukotriene B4 compared to neutrophils from rats fed a normal diet even though the arachidonate content was reduced by only 34%. Essential fatty acid-deficient neutrophils converted endogenous 5,8,11-eicosatrienoic acid to leukotriene A3 and its nonenzymatic degradation products, but little or no leukotriene B3 was formed. Neutrophils from rats fed a normal diet incubated with ionophore and exogenous 5,8,11-eicosatrienoate also produced leukotriene A3 and its nonenzymatic degradation products but little or no leukotriene B3. Exogenous 5,8,11-eicosatrienoate incubated with ionophore-stimulated normal neutrophils caused a dose-dependent inhibition of leukotriene A hydrolase resulting in diminished production of leukotriene B4 from endogenous arachidonate. Assays of leukotriene A hydrolase in the 10,000 X g supernatant fraction of a homogenate of RBL-1 cells revealed that a lipoxygenase metabolite of 5,8,11-eicosatrienoate rather than 5,8,11-eicosatrienoate itself is the inhibitor of leukotriene A hydrolase. Thus the finding that leukotriene B4 production by neutrophils from essential fatty acid-deficient rats is diminished out of proportion to the decrease in arachidonate content appears to be due to inhibition of leukotriene A hydrolase by a lipoxygenase metabolite.
- Subjects :
- 8,11,14-Eicosatrienoic Acid blood
Animals
Arachidonic Acids blood
Chemotaxis, Leukocyte drug effects
Leukotriene A4
Male
Mass Spectrometry
N-Formylmethionine Leucyl-Phenylalanine pharmacology
Rats
Rats, Inbred Strains
Spectrophotometry, Ultraviolet
Fatty Acids, Essential deficiency
Leukotriene B4 biosynthesis
Neutrophils metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 259
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6090446