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A filter paper sandwich method using small volumes of reagents for the detection of antigens electrophoretically transferred onto nitrocellulose.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1984 Dec 31; Vol. 75 (2), pp. 333-8. - Publication Year :
- 1984
-
Abstract
- A method is described which allows antigens electrophoretically 'blotted' onto nitrocellulose to be probed using small volumes (less than 0.3 ml) of antibody solutions. Reagent volumes required for this method are more than 10-fold lower than the minimum volumes attainable using either custom made or commercial incubation trays. The procedure is simple and economical and should be applicable to any situation where it is desirable to either conserve or reduce the amount of screening reagent used.
- Subjects :
- Antibodies, Monoclonal
Antigens, Surface analysis
Collodion
Electrophoresis, Polyacrylamide Gel methods
Female
Humans
Indicators and Reagents
Membrane Proteins analysis
Methods
Microvilli analysis
Paper
Placenta analysis
Pregnancy
Receptors, Cell Surface immunology
Receptors, Transferrin
Transferrin metabolism
Antigens analysis
Receptors, Cell Surface analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 75
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 6097613
- Full Text :
- https://doi.org/10.1016/0022-1759(84)90117-0