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Proenkephalin B (prodynorphin)-derived opioid peptides: evidence for a differential processing in lobes of the pituitary.
- Source :
-
Endocrinology [Endocrinology] 1984 Aug; Vol. 115 (2), pp. 662-71. - Publication Year :
- 1984
-
Abstract
- The distribution of peptides derived from the novel opioid peptide precursor proenkephalin B (prodynorphin) was studied in lobes of the pituitary with antibodies against alpha-neoendorphin (alpha-neo-E) beta-neoE, dynorphin (DYN)-(1-17), DYN-(1-8), and DYN B in combination with gel filtration and high pressure liquid chromatography. In the posterior pituitary, all five opioid peptides occurred in high and about equimolar concentrations, whereas putative precursor peptides were found in only minor quantities. In contrast, in the anterior pituitary immunoreactive (ir-) DYN-(1-17) and ir-DYN B consisted exclusively of a common precursor species with a mol wt of about 6000. Six thousand-dalton DYN may be comprised of the C-terminal portion of proenkephalin B, with the sequence of DYN-(1-17) at its N-terminus. Moreover, the major portions of ir-alpha-neo-E and ir-beta-neoE in the anterior pituitary were found to be of an apparent mol wt of 8000. These findings indicate a differential processing of the opioid peptide precursor proenkephalin B in the two lobes of the pituitary. The anterior pituitary seems to process proenkephalin B predominantly into high mol wt forms of neo-E and DYNs, whereas in the posterior pituitary proenkephalin B undergoes further proteolytic processing to the smaller opioid peptides alpha-neo-E, beta-neo-E, DYN-(1-17), DYN-(1-8), and DYN B. Thus, processing differences may enable the selective liberation of different (opioid) peptides with distinct biological properties from one precursor within different tissues.
- Subjects :
- Animals
Chromatography, High Pressure Liquid
Dynorphins
Enkephalin, Leucine analogs & derivatives
Enkephalin, Leucine metabolism
Male
Molecular Weight
Peptide Fragments metabolism
Pituitary Gland, Anterior metabolism
Pituitary Gland, Posterior metabolism
Radioimmunoassay
Rats
Rats, Inbred Strains
Tissue Distribution
Endorphins metabolism
Enkephalins metabolism
Pituitary Gland metabolism
Protein Precursors metabolism
beta-Endorphin analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0013-7227
- Volume :
- 115
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 6146512
- Full Text :
- https://doi.org/10.1210/endo-115-2-662