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A human Ia cytoplasmic determinant located on multiple forms of invariant chain (gamma, gamma 2, gamma 3).
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1984 Apr; Vol. 132 (4), pp. 1900-5. - Publication Year :
- 1984
-
Abstract
- An antigenic determinant present in the cytoplasm, but not on the surface membrane of human Ia+ cells, is defined by a monoclonal antibody (VIC-Y1) and is shown by immunoprecipitation and by NEPHGE to be expressed by Ia oligomers. Immunoprecipitations of cellfree translates and of purified Ia subunits indicate that the VIC-Y1 determinant is located on the Ia gamma-(invariant) chain, as well as on two other related molecules, provisionally termed gamma 2 and gamma 3. Within our experimental conditions, the three forms of gamma-chains co-precipitate exclusively with Ia oligomers. As detected by VIC-Y1 and in the limits of our assays, gamma-chains could not be found at the cell surface; their tissue distribution, determined by cytoplasmic indirect immunofluorescence with VIC-Y1, closely resembles that of Ia antigens, with the possible exception of acute lymphatic leukemia cells (Ia+, gamma-chain-).
- Subjects :
- Animals
Antibodies, Monoclonal immunology
B-Lymphocytes immunology
Electrophoresis, Polyacrylamide Gel
Epitopes genetics
Fluorescent Antibody Technique
Histocompatibility Antigens Class II analysis
Histocompatibility Antigens Class II isolation & purification
Humans
Mice
Mice, Inbred BALB C
Polymorphism, Genetic
Rabbits
Cytoplasm immunology
Epitopes immunology
Genes, MHC Class II
Histocompatibility Antigens Class II immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 132
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 6199417