Back to Search
Start Over
Lymphocyte induced macrophage cytotoxicity: characterization of the macrophage cytotoxicity-inducing lymphocyte.
- Source :
-
Immunobiology [Immunobiology] 1984 Jan; Vol. 166 (1), pp. 84-95. - Publication Year :
- 1984
-
Abstract
- The phenotype of lymphocytes, obtained from mice immunized with allogeneic tumor cells, with the capacity to induce macrophage cytotoxicity was determined. Macrophage cytotoxicity was induced, either by incubating the macrophages with Macrophage Arming Factor (MAF) containing supernatants of cultures of sensitized lymphocytes and tumor cells (arming) or by incubating the macrophages directly with sensitized lymphocytes and tumor cells (activation). The MAF producing or activating capacity of the lymphocytes was not only "triggered" by the sensitizing tumor cells but also by normal cells and other tumor cells bearing the H-2 determinants of the sensitizing tumor cell. The capacity to render macrophages cytotoxic was not reduced after treatment of the lymphocytes with mitomycin-C or treatment with anti-murine Ig and complement. This capacity of the lymphocytes was abrogated after treatment with anti-T-cell serum or anti-Thy 1.2 serum and complement. After treatment with anti-Lyt 1 or anti-Lyt 2 serum and complement, the activating capacity was significantly reduced and the MAF producing capacity of the lymphocytes abrogated. Mixing the Lyt 1 depleted and Lyt 2 depleted lymphocytes or addition of normal lymphocytes to the Lyt 1 depleted or Lyt 2 depleted populations did not restore the MAF producing and activating capacities. This indicated that the lymphocytes inducing macrophage cytotoxicity in this allogeneic system are Lyt-1+2+ T-lymphocytes, which do not need to divide prior to perform their action.
- Subjects :
- Animals
Antilymphocyte Serum
B-Lymphocytes immunology
Epitopes
Female
Lymphocyte Activation drug effects
Lymphoma, Non-Hodgkin immunology
Male
Mast-Cell Sarcoma immunology
Mice
Mice, Inbred AKR
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Mitomycins pharmacology
Neoplastic Stem Cells immunology
Phenotype
Plasmacytoma immunology
T-Lymphocytes immunology
Cytotoxicity, Immunologic
Lymphocytes immunology
Macrophage Activation
Subjects
Details
- Language :
- English
- ISSN :
- 0171-2985
- Volume :
- 166
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 6201434
- Full Text :
- https://doi.org/10.1016/S0171-2985(84)80146-1