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Radioimmunoassay for major internal structural protein (p21) of the bovine leukemia virus; antibodies detection and viruses identification.

Radioimmunoassay for major internal structural protein (p21) of the bovine leukemia virus; antibodies detection and viruses identification.

Authors :
Tarassishin LA
Zajac V
Altaner C
Popovic M
Grófová M
Grinenko NF
Altstein AD
Zhdanov VM
Source :
Neoplasma [Neoplasma] 1980; Vol. 27 (5), pp. 509-16.
Publication Year :
1980

Abstract

The major internal protein of bovine leukemia virus (BLV p24) was isolated using ion exchange chromatography on phosphocellulose and gel filtration. The specificity of the BLV p24 isolated was checked by both the radioimmunoprecipitation (RIP) and the competitive radioimmunoassay (RIA). No cross reactivity between BLV p25 and the mammalian viruses of type C and D and the retrovirus isolated from human myeloma cells RPMI8226 [8, 17] was detected. The analysis of 429 leukemia-suspected bovine blood sera resulted in the detection of 165 (38.5%) positive and 264 (61.5%) negative blood sera. A correlation of the results of radioimmunoprecipitation reaction of major internal protein p24 and immunodiffusion test on the glycoprotein antigen of BLV was observed.

Details

Language :
English
ISSN :
0028-2685
Volume :
27
Issue :
5
Database :
MEDLINE
Journal :
Neoplasma
Publication Type :
Academic Journal
Accession number :
6262668