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Radioimmunoassay for major internal structural protein (p21) of the bovine leukemia virus; antibodies detection and viruses identification.
Radioimmunoassay for major internal structural protein (p21) of the bovine leukemia virus; antibodies detection and viruses identification.
- Source :
-
Neoplasma [Neoplasma] 1980; Vol. 27 (5), pp. 509-16. - Publication Year :
- 1980
-
Abstract
- The major internal protein of bovine leukemia virus (BLV p24) was isolated using ion exchange chromatography on phosphocellulose and gel filtration. The specificity of the BLV p24 isolated was checked by both the radioimmunoprecipitation (RIP) and the competitive radioimmunoassay (RIA). No cross reactivity between BLV p25 and the mammalian viruses of type C and D and the retrovirus isolated from human myeloma cells RPMI8226 [8, 17] was detected. The analysis of 429 leukemia-suspected bovine blood sera resulted in the detection of 165 (38.5%) positive and 264 (61.5%) negative blood sera. A correlation of the results of radioimmunoprecipitation reaction of major internal protein p24 and immunodiffusion test on the glycoprotein antigen of BLV was observed.
- Subjects :
- Animals
Antibodies, Viral analysis
Cattle
Chromatography, Ion Exchange
Chromobox Protein Homolog 5
Cross Reactions
Glycoproteins immunology
Immunodiffusion
Leukemia Virus, Bovine immunology
Radioimmunoassay
Leukemia Virus, Bovine analysis
Leukemia Virus, Bovine classification
Retroviridae analysis
Retroviridae classification
Viral Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0028-2685
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Neoplasma
- Publication Type :
- Academic Journal
- Accession number :
- 6262668