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cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1981 May; Vol. 78 (5), pp. 2772-6. - Publication Year :
- 1981
-
Abstract
- mRNA coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability to hybridize specifically with mRNA coding for H-2K, D, or L antigens. One of these clones, pH-2d-1, carries a 1200-base-pair insert, comprising the noncoding region, including poly(A) at the 3' end and part of the coding region. A partial sequence of the latter region showed extensive homology with the known amino acid sequences of H-2Kb,Kk, and HLA-B7 antigens. From this comparison, it appears that the coding region extends from amino acid 133 in the second domain, through the third domain, to the cytoplasmic COOH-terminal region. A stretch of 24 hydrophobic or uncharged residues, located 31 amino acids from the COOH-terminal end, could represent the segment that spans the membrane. This is followed on the cytoplasmic side of the membrane by a cluster of basic amino acids and a possible phosphorylation site on a threonine residue.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
DNA Restriction Enzymes
Dogs
Escherichia coli genetics
Lymphoma immunology
Mice
Mice, Inbred DBA
Neoplasms, Experimental immunology
Nucleic Acid Hybridization
Plasmids
Rabbits
Reticulocytes metabolism
Cloning, Molecular
DNA, Recombinant immunology
H-2 Antigens genetics
Major Histocompatibility Complex
RNA, Messenger genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 78
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 6265910
- Full Text :
- https://doi.org/10.1073/pnas.78.5.2772