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Molecular cloning and comparative analyses of the genomes of simian sarcoma virus and its associated helper virus.

Molecular cloning and comparative analyses of the genomes of simian sarcoma virus and its associated helper virus.

Authors :
Gelmann EP
Wong-Staal F
Kramer RA
Gallo RC
Source :
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1981 Jun; Vol. 78 (6), pp. 3373-7.
Publication Year :
1981

Abstract

Closed circular viral DNA of simian sarcoma virus (SSV) and simian sarcoma-associated virus (SSAV) obtained from acutely infected dog cells was purified on preparative agarose gels, cleaved with EcoRI, and cloned in the phage lambda vector Charon 21A. The cloned 9-kilobase SSAV genome (B11) has the same restriction map as the bulk of the unintegrated linear SSAV DNA intermediate. Heteroduplex analysis between an SSV clone (lambda-C60) and an SSAV clone (lambda-B11) showed two substitution loops and one deletion loop. By using detailed restriction enzyme mapping and electron microscopic analysis, we showed that one of the substitution loops corresponds to an inversion of one of the two long terminal repeat units and adjacent cellular sequences in C60. The other substitution loop mapped close to the 3' long terminal repeat. At least part of this region was shown to contain SSV-specific sequences not shared by SSAV. The 1.9-kilobase deletion mapped at 3.5-5.5 kilobases of the linear SSAV genome, corresponding to most, if not all, of the pol gene.

Details

Language :
English
ISSN :
0027-8424
Volume :
78
Issue :
6
Database :
MEDLINE
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
6267588
Full Text :
https://doi.org/10.1073/pnas.78.6.3373