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The reconstitution reaction of Neurospora apotyrosinase.

Authors :
Beltramini M
Lerch K
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1983 Jan 14; Vol. 110 (1), pp. 313-9.
Publication Year :
1983

Abstract

The reconstitution of Neurospora apotyrosinase was studied in the presence of Cu(I) or Cu(II) ions. The kinetics and the mechanism of reactivation were found to differ markedly for the two metal ions. Thus the reconstitution with Cu(I) was found to be very fast and complete following an all or none process; in contrast the reaction with Cu(II) proved to be rather slow and incomplete with the two Cu(II) ions binding with different rate constants.

Subjects

Subjects :
Copper pharmacology
Kinetics
Macromolecular Substances
Spectrophotometry, Ultraviolet
Apoenzymes metabolism
Apoproteins metabolism
Catechol Oxidase metabolism
Monophenol Monooxygenase metabolism
Neurospora enzymology

Details

Language :
English
ISSN :
0006-291X
Volume :
110
Issue :
1
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
6404252
Full Text :
https://doi.org/10.1016/0006-291x(83)91297-4
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