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Distribution pattern of DNA template activity in the developing cerebellar cortex in rats and its alteration after transplacental administration of ethylnitrosourea.
- Source :
-
Acta pathologica japonica [Acta Pathol Jpn] 1984 May; Vol. 34 (3), pp. 489-97. - Publication Year :
- 1984
-
Abstract
- The ultracytochemical acridine orange(AO) method has been employed to demonstrate DNA template activity in the developing cerebellar cortex in rats and its alteration after transplacental administration of ethylnitrosourea (ENU). Electron microscopic studies revealed that AO binds to DNA exclusively within the active extended euchromatin portion of the cell nucleus of the proliferating external granular layer. No or only a few AO reaction products were visible in the non-proliferating cell nuclei of the internal granular layer and of Purkinje cells. With increasing cellular differentiation of granule cells in the cerebellar cortex the number of AO positive cells as well as the number of AO reaction products per cell showed a progressive decrease. A single i.p. injection of 50 mg/kg of ENU in rats on day 15 to 21 of gestation resulted in occasional cell damage to the proliferating external granule cells of developing cerebellar cortex at 4 and 7 days after birth. Damaged cells of the external granular layer were found to have no or only a small number of AO reaction products in their nuclei. In further development from 14 to 28 days the cerebellar cortex of offspring from ENU-treated mother showed no abnormality and exhibited no definite alteration in distribution pattern of AO reaction products.
- Subjects :
- Acridine Orange metabolism
Animals
Cerebellar Cortex drug effects
Cerebellar Cortex embryology
Female
Gestational Age
Histocytochemistry
Maternal-Fetal Exchange
Pregnancy
Rats
Rats, Inbred Strains
Templates, Genetic
Cerebellar Cortex metabolism
DNA metabolism
Ethylnitrosourea toxicity
Nitrosourea Compounds toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0001-6632
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Acta pathologica japonica
- Publication Type :
- Academic Journal
- Accession number :
- 6464748
- Full Text :
- https://doi.org/10.1111/j.1440-1827.1984.tb07577.x