[Spectrophotometric evaluation of diene conjugates].

Primary products of lipid peroxidation in liver tissue (diene conjugates) were extracted using various systems of solvents. In the system heptane-isopropanol 1:1 the diene conjugates were mainly extracted by the alcohol. The procedure for estimation of the tissue diene conjugates involved extraction with heptane-isopropanol, purification and spectrophotometric measurement of the extinction in the alcohol fraction.