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Ca2+ release in the endoplasmic reticulum of guinea pig peritoneal macrophages.
- Source :
-
Journal of biochemistry [J Biochem] 1983 Oct; Vol. 94 (4), pp. 1155-63. - Publication Year :
- 1983
-
Abstract
- Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca2+ was studied by the filtration method using 45Ca. The Ca2+ release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca2+ in the medium. The Ca2+ release was enhanced by caffeine, and suppressed by MgCl2. These phenomena are similar to the Ca2+-induced Ca2+ release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca2+ release from the endoplasmic reticulum, while it reportedly enhanced the Ca2+-induced Ca2+ release of the skeletal muscle. The threshold concentration of Ca2+ for the Ca2+-induced Ca2+ release was approximately 10(-8) M in the presence of 0.95 mM MgCl2 in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca2+-induced Ca2+ release.
- Subjects :
- Animals
Calcium pharmacology
Guinea Pigs
Kinetics
Macrophages drug effects
Macrophages ultrastructure
Microscopy, Electron
N-Formylmethionine Leucyl-Phenylalanine pharmacology
Neutrophils metabolism
Permeability
Saponins pharmacology
Sarcoplasmic Reticulum drug effects
Calcium metabolism
Endoplasmic Reticulum metabolism
Macrophages metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 94
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6654849
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a134460