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Metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocytes: studies with gas chromatography-mass spectrometry.
- Source :
-
Journal of neurochemistry [J Neurochem] 1984 Jan; Vol. 42 (1), pp. 283-6. - Publication Year :
- 1984
-
Abstract
- Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6689695
- Full Text :
- https://doi.org/10.1111/j.1471-4159.1984.tb09731.x