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Expression of the immunoglobulin C mu gene in mouse T and B lymphoid and myeloid cell lines.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1980 May; Vol. 77 (5), pp. 2876-80. - Publication Year :
- 1980
-
Abstract
- We have used cloned nucleotide sequences as probes to search for immunoglobulin RNA in T and B lymphoid and nonlymphoid tumor cell lines. Polyadenylated RNA from the cells was fractionated electrophoretically by size and fixed to diazobenzyloxymethyl-paper, and then immunoglobulin RNA species were revealed by hybridization with 32P-labeled cloned sequences for mu heavy chain and kappa light chain. kappa mRNA was detected only in B lymphoid lines known to synthesize kappa chains. No mu RNA was detectable in erythroleukemia, mastocytoma, or sarcoma cells. RNA bearing mu constant region (C mu) sequences, however, was detected in four of nine T lymphoma and four of five myeloid tumor cell lines tested, as well as in B lymphoma and "pre-B" Abelson lymphoma cells. In contrast to the single mu RNA species of 2.6 kilobases (kb) in a plasmacytoma, individual T lymphoma and myeloid lines yielded up to three discrete mu RNA species of apparent size 1.9, 2.2, and 3.0 kb, each different from the two mu RNA species in a B lymphoma line (2.4 and 2.7 kb) or the single species in "pre-B" lymphoma cells (2.9 kb). Both chromosomal complements of the C mu gene in STRij-4 T cells were found to be rearranged from their embryonic (germ line) context. The results suggest that the C mu gene functions not only in T cells, wherein the ability to recognize antigen is long established, but also in myeloid cells.
- Subjects :
- Animals
Cell Line
Genes
Granulocytes immunology
Immunoglobulin Light Chains genetics
Lymphoma immunology
Macrophages immunology
Mice
RNA, Messenger genetics
B-Lymphocytes immunology
Immunoglobulin Constant Regions genetics
Immunoglobulin Heavy Chains genetics
Immunoglobulin mu-Chains genetics
Immunoglobulins genetics
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 77
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 6771761
- Full Text :
- https://doi.org/10.1073/pnas.77.5.2876