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4-methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1983 Mar 16; Vol. 111 (2), pp. 484-9. - Publication Year :
- 1983
-
Abstract
- Enzymatic activity which catalyzes the synthesis of 4-methyleneglutamine from 4-methyleneglutamic acid + ammonia was detected in and partially purified from cotyledons of peanut seeds germinated 5 to 7 days. This activity was separated from glutamine and asparagine synthetases by ammonium sulfate precipitation and DEAE-cellulose chromatography. The enzyme is distinct from these other amide synthetases in its substrate specificity, lack of amide/hydroxylamine exchange, and use of ammonium ion as amide donor together with formation of AMP from ATP. The activity is quite labile in solution, but is retained as a precipitate in ammonium sulfate or when frozen in 12.5% glycerol at -77 degrees C. This activity might be responsible for catalyzing the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 111
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 6838571
- Full Text :
- https://doi.org/10.1016/0006-291x(83)90332-7