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Biological properties of human leukocyte interferon components.

Authors :
Paucker K
Dalton BJ
Törmä ET
Ogburn CA
Source :
The Journal of general virology [J Gen Virol] 1977 May; Vol. 35 (2), pp. 341-51.
Publication Year :
1977

Abstract

Human leukocyte interferon, purified approximately 1000-fold by affinity chromatography on immobilized anti-interferon globulins and SDS-Sephadex filtration, was resolved into one major and one minor component by adsorption chromatography on hydroxylapatite and electrophoresis in polyacrylamide gels. These components were indistinguishable in their capacity to protect bovine, porcine and murine cells, and the antiviral activities of both were equally susceptible to reduction by beta-mercaptoethanol. They were neutralized to the same degree of rabbit anti-leukocyte interferon but were not neutralized by rabbit antifibroblast interferon serum. Mice immunized with either component developed antibodies to both but failed to form antibodies against human fibroblast interferon. Our present evidence indicates that the two components posses at most only minor structural and antigenic dissimilarities.

Details

Language :
English
ISSN :
0022-1317
Volume :
35
Issue :
2
Database :
MEDLINE
Journal :
The Journal of general virology
Publication Type :
Academic Journal
Accession number :
69014
Full Text :
https://doi.org/10.1099/0022-1317-35-2-341