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Biological properties of human leukocyte interferon components.
- Source :
-
The Journal of general virology [J Gen Virol] 1977 May; Vol. 35 (2), pp. 341-51. - Publication Year :
- 1977
-
Abstract
- Human leukocyte interferon, purified approximately 1000-fold by affinity chromatography on immobilized anti-interferon globulins and SDS-Sephadex filtration, was resolved into one major and one minor component by adsorption chromatography on hydroxylapatite and electrophoresis in polyacrylamide gels. These components were indistinguishable in their capacity to protect bovine, porcine and murine cells, and the antiviral activities of both were equally susceptible to reduction by beta-mercaptoethanol. They were neutralized to the same degree of rabbit anti-leukocyte interferon but were not neutralized by rabbit antifibroblast interferon serum. Mice immunized with either component developed antibodies to both but failed to form antibodies against human fibroblast interferon. Our present evidence indicates that the two components posses at most only minor structural and antigenic dissimilarities.
- Subjects :
- Animals
Cattle
Cells, Cultured
Cytopathogenic Effect, Viral drug effects
Epitopes
Fibroblasts metabolism
Humans
Mercaptoethanol pharmacology
Mice
Species Specificity
Swine
Vesicular stomatitis Indiana virus drug effects
Vesicular stomatitis Indiana virus growth & development
Interferons biosynthesis
Interferons isolation & purification
Interferons pharmacology
Leukocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 69014
- Full Text :
- https://doi.org/10.1099/0022-1317-35-2-341