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Circular dichroism and fluorescence-detected circular dichroism of deoxyribonucleic acid and poly[d(A-C).d(G-T)] in ethanolic solutions: a new method for estimating circular intensity differential scattering.

Authors :
Reich C
Maestre MF
Edmondson S
Gray DM
Source :
Biochemistry [Biochemistry] 1980 Nov 11; Vol. 19 (23), pp. 5208-13.
Publication Year :
1980

Abstract

A method is presented for determining the circular dichroism (CD) of systems whose CD spectra contain contributions from CD differential scattering. The technique is shown to detect light over 4 pi steradians, and thus, for the first time, a complete correction for scattering is possible. The method is applied to ethanol-condensed DNA and poly[d(A-C).d(G-T)]. From the results obtained, the former are proposed to have an A-type secondary structure. The condensed polynucleotide particles are shown to exhibit behavior similar to that of cholesteric liquid crystals. CD difference spectra, obtained from the scattering corrections and showing the contributions to different sections of the scattering envelope, are displayed. It is asserted that these scattering patterns contain information about the tertiary structure of the condensed DNA particles studied.

Details

Language :
English
ISSN :
0006-2960
Volume :
19
Issue :
23
Database :
MEDLINE
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
7004483
Full Text :
https://doi.org/10.1021/bi00564a009