Glial fibrillary acidic protein synthesized in vitro using messenger RNA from jimpy mouse spinal cord.

Authors :: Bigbee JW
Eng LF
Source :: Brain research [Brain Res] 1982 Oct 14; Vol. 249 (2), pp. 383-6.
Publication Year :: 1982
Abstract: Glial fibrillary acidic (GFA) protein was synthesized in vitro in a rabbit reticulocyte lysate system programmed with messenger RNA (mRNA) extracted from Jimpy mouse spinal cord. It was identical in molecular weight and charge to that synthesized from normal mouse mRNA and GFA protein extracted from normal mouse cord. These data suggest that the Jimpy mutation does not affect the primary phenotypic expression of GFA protein.

Subjects :: Animals
Astrocytes metabolism
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation
Glial Fibrillary Acidic Protein
Intermediate Filament Proteins genetics
Male
Mice
Mice, Jimpy
Optic Nerve metabolism
Phenotype
Reticulocytes metabolism
Intermediate Filament Proteins biosynthesis
Myelin Sheath metabolism
RNA, Messenger metabolism
Spinal Cord metabolism

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