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Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1977 Nov 01; Vol. 146 (5), pp. 1234-45. - Publication Year :
- 1977
-
Abstract
- A simple procedure to enrich the antigen (keyhole limpet hemocyanin, KLH)-specific suppressor T cell was described. The suppressor T cell from KLH)-immunized mice specifically bound to the KLH-coated Sephadex G-200 column at 37 degrees C, and was eluted from the column by cold (0-4 degrees C) medium. The helper T cell did not bind to the column under the identical condition. The suppressor T cell thus obtained had 100 times as potent suppressor activity as the original spleen cells in in vivo and in vitro secondary antibody responses against a hapten coupled to KLH. This procedure also enriched the cells bearing I-J determinants and Lyt-2,3 alloantigens, allowing us to study the phenotypic expressions on the suppressor T cell by direct serological procedures as well as by the use of the fluorescence activated cell sorter. Parallel functional and serological analyses indicated that the antigen-specific suppressor T cell belongs to a population of I-J+, Lyt-2+,3+ and Fc R- T cells.
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 146
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 72775
- Full Text :
- https://doi.org/10.1084/jem.146.5.1234