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Energy reserve and cation composition of irreversibly sickled cells in vivo.

Authors :
Glader BE
Lux SE
Muller-Soyano A
Platt OS
Propper RD
Nathan DG
Source :
British journal of haematology [Br J Haematol] 1978 Dec; Vol. 40 (4), pp. 527-32.
Publication Year :
1978

Abstract

Cation composition, cellular hydration, and adenosinetriphosphate (ATP) content were measured in irreversible sickle cells (ISC's) separated from the blood of patients with sickle cell anaemia. Total monovalent cation (Na+ + K+) content was markedly reduced in ISC's and this largely was due to cell K+ depletion. Corresponding to the reduced cation content, cells were dehydrated as indicated by a reduced mean cell volume. ISC's also appeared to be grossly depleted of ATP. These biochemical characteristics allow us to expand the definition of ISC's beyond morphologic characteristics. In addition, these chemical alterations provide a means for elucidating the mechanism of ISC production in vitro.

Details

Language :
English
ISSN :
0007-1048
Volume :
40
Issue :
4
Database :
MEDLINE
Journal :
British journal of haematology
Publication Type :
Academic Journal
Accession number :
728369
Full Text :
https://doi.org/10.1111/j.1365-2141.1978.tb05828.x