Processing and activation of recombinant mouse mastocytoma histidine decarboxylase in the particulate fraction of Sf9 cells by porcine pancreatic elastase.

Authors :: Tanaka S
Fukui T
Yamamoto J
Shima Y
Kume T
Ohgo M
Ichikawa A
Source :: Biochimica et biophysica acta [Biochim Biophys Acta] 1995 Nov 15; Vol. 1253 (1), pp. 9-12.
Publication Year :: 1995
Abstract: Mature 53 kDa histidine decarboxylase (HDC) peptide is produced from a precursor 74 kDa peptide. The mechanism of specific cleavage by processing enzyme is unknown. Using the recombinant mouse 74 kDa HDC, we found that porcine pancreatic elastase specifically converted the inactive 74 kDa HDC to its active form of 53 kDa HDC.

Subjects :: Animals
Cell Line
Chromatography, Agarose
Endopeptidases metabolism
Histidine Decarboxylase chemistry
Histidine Decarboxylase isolation & purification
Immunoblotting
Mast-Cell Sarcoma enzymology
Mice
Molecular Weight
Pancreas enzymology
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins isolation & purification
Recombinant Fusion Proteins metabolism
Solubility
Swine
Histidine Decarboxylase metabolism
Pancreatic Elastase metabolism
Protein Processing, Post-Translational

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