Back to Search
Start Over
Molecular basis of cross-reactivity among allergen-specific human T cells: T-cell receptor V alpha gene usage and epitope structure.
- Source :
-
Immunology [Immunology] 1994 Jan; Vol. 81 (1), pp. 15-20. - Publication Year :
- 1994
-
Abstract
- Cross-reactivities between the major grass pollen allergens, at the level of T-cell recognition was examined employing several Lolium perenne I (Lol p I)-specific human T-cell clones. Nine of these Lol p I-specific T-cell clones exhibited cross-recognition of the recombinant Poa pratensis IX (Poa p IX) allergen, rKBG7.2, indicating that these two major antigens of a grass pollen share T-cell epitopes. Furthermore, proliferative responses of two other T-cell clones demonstrated that individual allergens of diverse grass pollens also possess common T-cell epitopes. Examination of the T-cell receptor (TcR) V alpha genes of these T-cell clones indicated that these cloned cells utilized distinct J alpha genes and that nine out of 10 clones possessed V alpha 13 gene. Furthermore, sequence comparisons of several allergenic molecules indicated that this cross-reactivity may be due to the presence of epitope(s) with structure(s) similar to the major T-cell epitope of Poa p IX allergens. Taken together, these results suggest for the first time that the major grass pollen allergens share cross-reacting T-cell epitope(s), and that this cross-reactivity is due to the structural homologies among allergens and restricted usage of TcR V alpha genes.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cell Division immunology
Cross Reactions immunology
DNA chemistry
Humans
Molecular Sequence Data
Poaceae immunology
Polymerase Chain Reaction
Allergens immunology
Epitopes chemistry
Pollen immunology
Receptors, Antigen, T-Cell, alpha-beta genetics
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 81
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7510663