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Coengagement of CD2 with LFA-1 or VLA-4 by bispecific ligand fusion proteins primes T cells to respond more effectively to T cell receptor-dependent signals.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 1994 Oct; Vol. 56 (4), pp. 444-52. - Publication Year :
- 1994
-
Abstract
- To examine the effects of ligand engagement and accessory molecule juxtaposition on T cell receptor (TCR) signaling, we prepared LFA-3/ICAM-1 Rg and LFA-3/VCAM-1 Rg bispecific immunoglobulin fusion proteins (Rg, recombinant globulin). These novel fusion proteins allowed us to examine the effects of ligand driven co-engagement of T cell proteins CD2 and LFA-1 or CD2 and VLA-4 on TCR-dependent mobilization of intracellular Ca2+. We observed that preincubation of resting T cells with LFA-3/ICAM-1 Rg or LFA-3/VCAM-1 Rg fusion proteins resulted in significantly enhanced mobilization of intracellular Ca2+ following TCR-accessory molecule cross-linking relative to T cells preincubated with each of the monospecific Rgs alone or with combinations of the monospecific Rg fusion proteins. In addition, such coengagement stimulated TCR-dependent activation and tyrosine phosphorylation of phospholipase C gamma 1 (PLC gamma 1). These results suggest that when T cells interact with antigen presenting cells the engagement of multiple cell adhesion molecules such as CD2, LFA-1, and VLA-4 primes the T cell to respond more effectively to signals delivered through the TCR.
- Subjects :
- Antibodies, Bispecific
Antigens, CD physiology
CD3 Complex physiology
CD58 Antigens
Calcium metabolism
Humans
Intercellular Adhesion Molecule-1 physiology
Membrane Glycoproteins physiology
Receptor Aggregation
Recombinant Fusion Proteins
Signal Transduction
CD2 Antigens physiology
Lymphocyte Function-Associated Antigen-1 physiology
Receptors, Antigen, T-Cell physiology
Receptors, Very Late Antigen physiology
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 56
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 7523557
- Full Text :
- https://doi.org/10.1002/jlb.56.4.444