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Induction of eosinophilic granules, nonspecific esterase activity and CD14 expression in the human eosinophilic leukemia cell line, EOL-1.
- Source :
-
Hematological oncology [Hematol Oncol] 1994 May-Jun; Vol. 12 (3), pp. 129-39. - Publication Year :
- 1994
-
Abstract
- We examined the expression of eosinophilic granules, esterase activity and CD14 in a human eosinophilic cell line, EoL-1. Unstimulated EoL-1 cells were weakly positive for nonspecific esterase, but negative for surface CD14, and contained a few eosinophilic granule-positive cells. A combination of G-CSF and TNF-alpha increased the eosinophilic granule-containing cells, but failed to increase esterase activity or CD14 expression. IFN-gamma alone or in combination with TNF-alpha enhanced nonspecific esterase activity but failed to induce CD14 expression or increase eosinophilic granule-containing cells. dbcAMP increased eosinophilic granule-containing cells, nonspecific esterase activity and CD14 expression. Specific esterase activity was not detected in any circumstances. EoL-1 cells fractionated by density gradients or CD14 expression showed nonspecific esterase activity and CD14 expression in both the eosinophilic granule-positive and negative cell populations. Forskolin and butyrate had a synergistic effect on CD14 induction and protein kinase A was suggested to play a role in dbcAMP-induced CD14 expression. A protein kinase C activator, phorbol 12-myristate 13-acetate, did not increase eosinophilic granules, nonspecific esterase activity or CD14 expression in EoL-1 cells. The results show that EoL-1 cells can express nonspecific esterase and CD14, but the expression is not necessarily restricted to cells which have differentiated into the monocyte/macrophage lineage.
- Subjects :
- Antigens, CD analysis
Antigens, Differentiation, Myelomonocytic analysis
Bucladesine pharmacology
Cell Differentiation
Cell Line
Colforsin pharmacology
Cytoplasmic Granules ultrastructure
Granulocyte Colony-Stimulating Factor pharmacology
Humans
Hypereosinophilic Syndrome enzymology
Hypereosinophilic Syndrome immunology
Interferon-gamma pharmacology
Lipopolysaccharide Receptors
Monocytes
Tumor Necrosis Factor-alpha pharmacology
Esterases analysis
Hypereosinophilic Syndrome pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0278-0232
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Hematological oncology
- Publication Type :
- Academic Journal
- Accession number :
- 7525448
- Full Text :
- https://doi.org/10.1002/hon.2900120305