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Activation of central GABAA-but not of GABAB-receptors rapidly reduces pituitary LH release and GnRH gene expression in the preoptic/anterior hypothalamic area of ovariectomized rats.
- Source :
-
Neuroendocrinology [Neuroendocrinology] 1995 Jun; Vol. 61 (6), pp. 655-62. - Publication Year :
- 1995
-
Abstract
- gamma-Aminobutyric acid (GABA) exerts an inhibitory action on gonadotropin-releasing hormone (GnRH) release from the hypothalamus. In vivo, this inhibitory action appears to be mediated via the GABAA receptor since in ovariectomized (ovx) rats and sheep direct application of muscimol (MUS), a GABAA agonist, into the preoptic area (POA), the site were the GnRH cell bodies are located, caused an immediate reduction of LH release. This effect may be the result of an inhibition of GnRH release but also GnRH biosynthesis may be affected. Using competitive reverse transcription-polymerase chain reaction (RT-PCR) we now addressed the question, whether an acute inhibition of the GnRH pulse generator in ovx rats by GABA involves reduction of GnRH biosynthesis as determined by GnRH mRNA levels in micropunches of the POA. To activate either the GABAA or GABAB receptor, we injected intraventricularly (icv) MUS or baclofen (BAC). Intracerebroventricular injection of 10 nmol MUS caused a rapid and lasting inhibition of LH release from about 7.5 ng/ml (pretreatment value) to approximately 1.5 ng/ml. Neither application of BAC or saline (control injections) affected LH secretion. Two hours after icv injections, rats were decapitated and GnRH mRNA levels were determined. MUS induced a pronounced decrease of GnRH levels in the POA (control rats: 2.26 pg GnRH mRNA; MUS-treated rats: 0.85 pg, n = 10/group). BAC was without any effect on GnRH mRNA levels. Thus, we confirm the inhibitory action of GABA on LH release in vivo which is exerted via the A-subtype of the receptor.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Baclofen pharmacology
Female
Gene Expression
Hypothalamus, Anterior metabolism
Muscimol pharmacology
Ovariectomy
Pituitary Gland drug effects
Polymerase Chain Reaction
Preoptic Area drug effects
RNA, Messenger biosynthesis
RNA-Directed DNA Polymerase metabolism
Rats
GABA-A Receptor Agonists
GABA-B Receptor Agonists
Gonadotropin-Releasing Hormone genetics
Luteinizing Hormone blood
Pituitary Gland metabolism
Preoptic Area metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0028-3835
- Volume :
- 61
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Neuroendocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 7544879
- Full Text :
- https://doi.org/10.1159/000126892