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Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector.

Authors :
Kinner E
Pocta D
Ströer S
Schmieger H
Source :
FEMS microbiology letters [FEMS Microbiol Lett] 1994 May 15; Vol. 118 (3), pp. 283-9.
Publication Year :
1994

Abstract

The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.

Details

Language :
English
ISSN :
0378-1097
Volume :
118
Issue :
3
Database :
MEDLINE
Journal :
FEMS microbiology letters
Publication Type :
Academic Journal
Accession number :
7545934
Full Text :
https://doi.org/10.1111/j.1574-6968.1994.tb06842.x