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Instability of Rickettsia prowazekii RNA polymerase-promoter complexes.
- Source :
-
Journal of bacteriology [J Bacteriol] 1995 Nov; Vol. 177 (21), pp. 6301-3. - Publication Year :
- 1995
-
Abstract
- The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.
- Subjects :
- Coenzymes metabolism
DNA, Bacterial metabolism
Heparin pharmacology
Osmolar Concentration
Promoter Regions, Genetic
Protein Binding
Rickettsia prowazekii enzymology
Sigma Factor metabolism
DNA-Directed RNA Polymerases metabolism
Rickettsia prowazekii genetics
Transcription, Genetic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 177
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 7592400
- Full Text :
- https://doi.org/10.1128/jb.177.21.6301-6303.1995