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Activation of protein kinase C by trimethyltin: relevance to neurotoxicity.
- Source :
-
Journal of neurochemistry [J Neurochem] 1995 Nov; Vol. 65 (5), pp. 2338-43. - Publication Year :
- 1995
-
Abstract
- The differentiated PC12 cell neuronal model was used to determine the effect of trimethyltin (TMT) on protein kinase C (PKC). Cells treated with 5-20 microM TMT showed a partial and sustained PKC translocation within 30 min and persisted over a 24-h period. TMT treatment was accompanied by a low level of PKC down-regulation over 24 h, which was small compared with that produced by phorbol esters. Confocal imaging of differentiated PC12 cells showed that PKC translocates to the plasma membrane and the translocation is blocked by the PKC inhibitor chelerythrine (1 microM). Phorbol myristate-induced PKC down-regulation or inhibition with chelerythrine provided protection against TMT-induced cytotoxicity. It was concluded that TMT-induced PKC translocation and activation contribute to the cytotoxicity of TMT in differentiated PC12 cells.
- Subjects :
- Alkaloids
Animals
Benzophenanthridines
Blotting, Western
Cell Death
Cell Differentiation
Enzyme Activation
Immunohistochemistry
Microscopy, Confocal
PC12 Cells drug effects
PC12 Cells metabolism
Phenanthridines pharmacology
Protein Kinase C antagonists & inhibitors
Rats
Tissue Distribution
Neurotoxins pharmacology
Protein Kinase C metabolism
Trimethyltin Compounds pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 65
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7595523
- Full Text :
- https://doi.org/10.1046/j.1471-4159.1995.65052338.x