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Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types.
- Source :
-
Genes & development [Genes Dev] 1993 May; Vol. 7 (5), pp. 749-59. - Publication Year :
- 1993
-
Abstract
- The c-Myb transcription factor regulates the differentiation of immature erythroid, lymphoid, and myeloid cells, although only the latter cells become transformed by the v-myb oncogene. These are also the only cells that express the Myb-regulated gene mim-1, suggesting that Myb requires tissue-specific, cooperating factors to activate such genes. Here, we investigated the tissue-specific regulation of the mim-1 promoter and found that it not only contains binding sites for Myb but also for NF-M, a myeloid-specific transcription factor that probably corresponds to mammalian C/EBP beta. Both types of binding sites were found to be required for full activity of the promoter. Remarkably, ectopic coexpression of Myb and NF-M proteins in erythroid cells or fibroblasts was sufficient to induce endogenous markers of myeloid differentiation, like the mim-1 and lysozyme genes. Our results indicate that c-Myb and NF-M proteins act as a bipartite, combinatorial signal that regulates the expression of myeloid-specific genes, even in heterologous cell types.
- Subjects :
- Animals
Base Sequence
CCAAT-Enhancer-Binding Proteins
Cell Line
Chickens
DNA-Binding Proteins physiology
Fibroblasts metabolism
Gene Expression Regulation physiology
Genes physiology
Hematopoiesis genetics
Molecular Sequence Data
Muramidase biosynthesis
Muramidase genetics
Mutagenesis, Site-Directed
Nuclear Proteins physiology
Poly A analysis
Poly A isolation & purification
Polymerase Chain Reaction
Proto-Oncogene Proteins physiology
Proto-Oncogene Proteins c-myb
Quail
RNA analysis
RNA isolation & purification
RNA, Messenger
Transcription Factors genetics
Transcription Factors physiology
Transcription, Genetic
Transfection
Acetyltransferases
DNA-Binding Proteins genetics
Nuclear Proteins genetics
Promoter Regions, Genetic
Proteins genetics
Proto-Oncogene Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0890-9369
- Volume :
- 7
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 7684005
- Full Text :
- https://doi.org/10.1101/gad.7.5.749