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Partial purification of cross-protection factor(s) from Pasteurella multocida.
- Source :
-
Avian diseases [Avian Dis] 1994 Oct-Dec; Vol. 38 (4), pp. 778-89. - Publication Year :
- 1994
-
Abstract
- Membrane-associated cross-protection factor(s) (CPF) of in vivo-grown Pasteurella multocida were solubilized by detergent and partially purified by sequential chromatography on ion exchange, gel filtration, and hydroxyapatite columns. The CPF activity was determined by challenge of turkeys vaccinated with different chromatography fractions and challenge of poults passively immunized with serum from the same vaccinated turkeys. Analyses of different chromatography fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots suggested that proteins in the range of 59-65 kDa and a protein of 39-kDa molecular mass were associated with cross-protection. Although CPF activity was found to be associated with protein-containing material, treatment with periodate diminished cross-protection, indicating that carbohydrate determinants may also contribute to immune cross-protection.
- Subjects :
- Animals
Antigens, Bacterial isolation & purification
Chromatography veterinary
Cross Reactions
Pasteurella Infections prevention & control
Pasteurella multocida chemistry
Antigens, Bacterial immunology
Bacterial Vaccines
Pasteurella Infections veterinary
Pasteurella multocida immunology
Poultry Diseases prevention & control
Turkeys
Subjects
Details
- Language :
- English
- ISSN :
- 0005-2086
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Avian diseases
- Publication Type :
- Academic Journal
- Accession number :
- 7702511