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Superoxide from glucose oxidase or from nitroblue tetrazolium?
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 1995 Apr 20; Vol. 318 (2), pp. 408-10. - Publication Year :
- 1995
-
Abstract
- Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O2- production which does not occur in their absence when O2 is the sole electron acceptor.
Details
- Language :
- English
- ISSN :
- 0003-9861
- Volume :
- 318
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 7733670
- Full Text :
- https://doi.org/10.1006/abbi.1995.1247