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Superoxide from glucose oxidase or from nitroblue tetrazolium?

Authors :
Liochev SI
Fridovich I
Source :
Archives of biochemistry and biophysics [Arch Biochem Biophys] 1995 Apr 20; Vol. 318 (2), pp. 408-10.
Publication Year :
1995

Abstract

Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O2- production which does not occur in their absence when O2 is the sole electron acceptor.

Details

Language :
English
ISSN :
0003-9861
Volume :
318
Issue :
2
Database :
MEDLINE
Journal :
Archives of biochemistry and biophysics
Publication Type :
Academic Journal
Accession number :
7733670
Full Text :
https://doi.org/10.1006/abbi.1995.1247