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Osteoclast formation from human cord blood mononuclear cells co-cultured with mice embryonic metatarsals in the presence of M-CSF.
- Source :
-
Bone [Bone] 1995 Jan; Vol. 16 (1), pp. 171-7. - Publication Year :
- 1995
-
Abstract
- Investigating the potentiality of cord monocytes to differentiate toward osteoclast-like cells (OCL) in vitro, we previously reported that in the presence of 1,25(OH)2 vitamin D3 (1,25-(OH)2D3), multinucleated-cells generated by cord monocyte cultures though displaying morphological features of OCL failed to resorb devitalized bones. We thus hypothesized that full differentiation of cord monocytes toward bone-resorbing cells may require the presence of factors released from and/or direct interactions with living osteogenic cells. In the present study, we tested these hypotheses using two culture systems supporting the development of bone-resorbing cells in the presence of bone matrix. First, cord mononuclear cells were co-cultured with murine fetal metatarsals depleted of osteoclast progenitor cells (stripped metatarsals) in the presence of 1,25-(OH)2D3. We found that cord mononuclear cells failed to differentiate toward OCL as indicated by the absence of the release of 45Ca previously incorporated in fetal bones and by the absence of formation of TRAP-positive (TRAP[+]) multinucleated cells which have invaded mineralized cartilage during the co-culture period. In the same model, we then investigated the effect of some soluble factors known as stimulators of osteoclast differentiation. Whereas exogenous rhIL6 and rhIL3 were ineffective in this assay, rhM-CSF consistently increased both the number of TRAP(+) multinucleated cells inside the mineralized cartilage and the release of 45Ca into the culture media. The effects of rhM-CSF were time-dependent reaching the maximum after 3 weeks of culture.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Calcitonin pharmacology
Cell Adhesion drug effects
Cell Differentiation drug effects
Cells, Cultured
Embryo, Mammalian cytology
Embryo, Mammalian drug effects
Embryonic and Fetal Development drug effects
Histocytochemistry
Humans
Leukocytes, Mononuclear cytology
Metatarsal Bones cytology
Metatarsal Bones embryology
Mice
Osteoclasts cytology
Fetal Blood cytology
Leukocytes, Mononuclear drug effects
Macrophage Colony-Stimulating Factor pharmacology
Metatarsal Bones drug effects
Osteoclasts drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 8756-3282
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bone
- Publication Type :
- Academic Journal
- Accession number :
- 7742078
- Full Text :
- https://doi.org/10.1016/s8756-3282(94)00026-3