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Evidence for a high molecular weight pre-robustoxin molecule in the venom of the male Sydney funnel-web spider (Atrax robustus).

Authors :
Collins SP
Comis A
Tyler MI
Marshall M
Howden ME
Source :
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology [Comp Biochem Physiol C Pharmacol Toxicol Endocrinol] 1995 Jan; Vol. 110 (1), pp. 89-93.
Publication Year :
1995

Abstract

Robustoxin is the lethal polypeptide toxin in Atrax robustus venom. A monoclonal antibody was produced using synthetic, unfolded robustoxin conjugated to keyhole limpet haemocyanin as the immunogen. This monoclonal antibody did not protect newborn mice against challenge with the crude venom of the male Sydney funnel-web spider, but did slightly prolong their survival time. Western blotted crude venom of the male Sydney funnel-web spider showed two monoclonal antibody binding bands. One band at low M(r) corresponded to robustoxin (M(r) 4854), while the other higher M(r) band (approximately 37,000) may be due to a pre-robustoxin molecule.

Details

Language :
English
ISSN :
1367-8280
Volume :
110
Issue :
1
Database :
MEDLINE
Journal :
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology
Publication Type :
Academic Journal
Accession number :
7749608
Full Text :
https://doi.org/10.1016/0742-8413(95)93865-t