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Adenylate energy charge of rat and human cultured hepatocytes.
Adenylate energy charge of rat and human cultured hepatocytes.
- Source :
-
In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 1994 Sep; Vol. 30A (9), pp. 609-14. - Publication Year :
- 1994
-
Abstract
- A simple and rapid method for the assay of adenine nucleotides (ATP, ADP, and AMP) was established to evaluate the adenylate energy charge (ATP+ADP/2)/(ATP+ADP+AMP) of cultured hepatocytes. The effects of inhibitors of glycolysis, fatty acid oxidation, or oxidative phosphorylation on the energy charge were examined. The energy charges of cultured hepatocytes in rats and human were almost identical and were maintained at a high level between 6 and 24 h after changing the media (rat: 0.908 +/- 0.008 n = 9, human: 0.918 +/- 0.014 n = 6, mean +/- SD). Inhibition of glycolysis with sodium fluoride or oxidative phosphorylation with antimycin A irreversibly reduced both the adenine nucleotide contents and the energy charge. However, the inhibition of fatty acid oxidation with 2-tetradecylglycidic acid did not affect the nucleotide contents, and the energy charge only decreased transiently to recover within 8 h. When the inhibitor of oxidative phosphorylation was removed, the recovery in the energy charge preceded the recovery in the adenine nucleotide contents. These findings suggest that the adenylate energy charge is a more sensitive measure of the changes in energy metabolism than the adenine nucleotide contents. Furthermore, energy charge regulates adenine nucleotide contents in cultured hepatocytes. It is important to confirm that the high energy charge of the cultured hepatocytes is maintained when these cells are used for metabolic studies.
- Subjects :
- Adenosine Diphosphate metabolism
Adenosine Monophosphate metabolism
Adenosine Triphosphate metabolism
Aged
Animals
Antimycin A pharmacology
Cells, Cultured
Chromatography, High Pressure Liquid
Cold Temperature
Epoxy Compounds pharmacology
Fatty Acids metabolism
Fatty Acids pharmacology
Glycolysis drug effects
Humans
Liver drug effects
Liver metabolism
Male
Oxidation-Reduction
Oxidative Phosphorylation drug effects
Rats
Rats, Wistar
Sodium Fluoride pharmacology
Adenosine Diphosphate analysis
Adenosine Monophosphate analysis
Adenosine Triphosphate analysis
Energy Metabolism
Liver chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1071-2690
- Volume :
- 30A
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- In vitro cellular & developmental biology. Animal
- Publication Type :
- Academic Journal
- Accession number :
- 7820312
- Full Text :
- https://doi.org/10.1007/BF02631260