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Localization of hepatitis C virus antigens in liver and skin tissues of chronic hepatitis C virus-infected patients with mixed cryoglobulinemia.
- Source :
-
Hepatology (Baltimore, Md.) [Hepatology] 1995 Feb; Vol. 21 (2), pp. 305-12. - Publication Year :
- 1995
-
Abstract
- Skin and/or liver biopsy specimens were obtained from the following patients: 15 anti-hepatitis C virus (HCV), HCV RNA-positive patients and 3 anti-HCV, HCV RNA-negative patients with type II mixed cryoglobulinemia (MC); 7 anti-HCV, HCV RNA-positive patients with chronic active liver disease (CALD); 5 anti-HCV, HCV RNA-negative patients with noncryoglobulinemic vasculitis; and 7 anti-HCV, HCV RNA-negative patients with lichen ruber planus. A pool of murine monoclonal antibodies (MAbs) developed against c22-3, c33c, and c100-3 proteins was used to detect HCV-related antigens (Ags) by indirect immunohistochemistry. Acid electroelution (AEE) of tissue sections was performed to enhance the sensitivity of the immunohistochemical method. In anti-HCV-positive MC patients, specific HCV-related Ags were detected in the small vessels of the skin and in the cytoplasm of hepatocytes. Prior AEE of biopsy sections allowed detection of HCV Ags in 6 of 15 (40%) skin biopsy and in 9 of 14 (64.3%) liver biopsy specimens. HCV immunoreactive deposits in the skin displayed two immunohistochemical patterns: (1) coarse intraluminal material associated with dermal inflammatory infiltrates and intravascular deposition of eosinophilic hyaline material; and (2) reactivity confined to the vessel wall in the context of an apparently normal tissue. Immunoglobulin (Ig) G and IgM deposition in the skin showed immunohistochemical features comparable with those found for HCV Ag deposits.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Adult
Base Sequence
Biopsy
Chronic Disease
Female
Hepacivirus genetics
Hepatitis C immunology
Hepatitis C pathology
Hepatitis C virology
Humans
Liver chemistry
Liver pathology
Male
Middle Aged
Molecular Sequence Data
RNA, Viral blood
Sensitivity and Specificity
Skin chemistry
Skin pathology
Antigens, Viral analysis
Cryoglobulinemia complications
Hepacivirus immunology
Hepatitis C complications
Subjects
Details
- Language :
- English
- ISSN :
- 0270-9139
- Volume :
- 21
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Hepatology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 7843698