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V delta 5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity.
- Source :
-
Immunology [Immunology] 1994 Feb; Vol. 81 (2), pp. 240-6. - Publication Year :
- 1994
-
Abstract
- The purpose of this paper was to study the gamma delta T-cell receptor repertoire and target specificity following stimulation of peripheral T cells of BALB/c mice with autologous or bacterial ligands. The expression of V gamma and V delta chain families in T cells that had been expanded by stimulation in syngeneic mixed lymphocyte culture or with purified protein derivative (PPD) was determined by the semiquantitative DNA polymerase chain reaction (PCR) method. Responder T cells to either of these stimuli strongly expressed both V delta 5 and V delta 6 genes. However, addition of the ML 30 anti-murine heat shock protein (hsp) 60 monoclonal antibody (mAb) to the cell culture selectively inhibited only the expansion of V delta 5 T cells. A V delta 5 T-cell hybridoma (KMT-5), which recognized syngeneic splenic and fibrosarcoma Meth A cells but not allogeneic cells, was produced by cell fusion from autoreactive blast cells. Incubation of the KMT-5 hybridoma in the presence of ML 30 antibody blocked the stimulation of interleukin-2 (IL-2) secretion by syngeneic target cells. It was also found that the DNA of KMT-5 hybridoma and of the autoreactive gamma delta T cells contained the BALB/c invariant delta (BID) chain sequence. It is concluded from these results that BALB/c peripheral V delta 5 T cells recognize an autologous hsp 60 target specificity in a V delta-gene restricted manner. We also propose that T cells of this V gene family may be involved in the immune surveillance of certain tumours and intracellular infections.
- Subjects :
- Animals
Base Sequence
Blotting, Southern
Chaperonin 60
DNA chemistry
Female
Gene Expression immunology
Immunoglobulin Variable Region genetics
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, gamma-delta analysis
Tuberculin immunology
Heat-Shock Proteins immunology
Receptors, Antigen, T-Cell, gamma-delta genetics
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 81
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7908891