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[Inhibition of oxidative phosphorylation in Crithidia fasciculata and Trypanosoma cruzi by lipophilic o-quinones and nifurtimox].
- Source :
-
Revista Argentina de microbiologia [Rev Argent Microbiol] 1994 Apr-Jun; Vol. 26 (2), pp. 72-86. - Publication Year :
- 1994
-
Abstract
- ATP and ADP levels were determined in Crithidia fasciculata and Trypanosoma cruzi. The nucleotide levels in crithidia or epimastigotes at the stationary phase of growth were, in nmol/10(8) cells, 15-40, and 3-7, for ATP and ADP, respectively. Incubation with the lipophilic o-naphthoquinones CG 8-935, CG 9-442 and CG 10-248 or the anti-chagasic nitrofuran nifurtimox, significantly decreased ATP level, with non-significant variations of the ADP level. The kinetics of ATP variation showed an initial 1-2 h lag and the diminution of the ATP level reached maximum value after 4-6 h incubation. Addition of L-glutamate or D-glucose as energy sources produced 2- or 3-fold increase of ATP level, after incubation the protozoa for 4-6 h with the corresponding substrates. Quinones and nifurtimox strongly depressed D-glucose or L-glutamate effects. Buthionine sulfoximine an inhibitor of glutathione biosynthesis, enhanced the effect of nifurtimox on ATP level in Crithidia fasciculata. It is concluded that by inhibiting ATP synthesis, either directly or-through oxygen radicals, the assayed drugs produced their cytotoxic action.
- Subjects :
- Adenosine Diphosphate metabolism
Adenosine Triphosphate metabolism
Animals
Crithidia fasciculata metabolism
Naphthoquinones
Trypanosoma cruzi metabolism
Crithidia fasciculata drug effects
Nifurtimox pharmacology
Oxidative Phosphorylation drug effects
Quinones pharmacology
Trypanosoma cruzi drug effects
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0325-7541
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Revista Argentina de microbiologia
- Publication Type :
- Academic Journal
- Accession number :
- 7938505