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Permeabilization of cells of hemopoietic origin by extracellular ATP4-: elimination of osteoclasts, macrophages, and their precursors from isolated bone cell populations and fetal bone rudiments.
- Source :
-
Calcified tissue international [Calcif Tissue Int] 1994 Aug; Vol. 55 (2), pp. 141-50. - Publication Year :
- 1994
-
Abstract
- Skeletal tissues contain, apart from cells of the osteogenic and chondrogenic lineage, cells of hemopoietic origin, e.g., macrophages, osteoclasts, and their precursors. In the present study we examined the sensitivity for extracellular ATP4- of the above-mentioned cell types in freshly isolated, bone-derived cell populations and in explanted fetal metatarsal bones. Cells of hemopoietic origin reacted to the presence of ATP4- with an increased permeability for impermeant cytotoxic molecules, e.g., ethidium bromide (EB), thiocyanate (KSCN), and an increased non-ion selective membrane conductance. As a consequence, these cells could be killed by a short treatment with adenosine-5' triphosphate (ATP)+KSCN. On the other hand, cells of nonhemopoietic origin (e.g., osteoblasts, chondrocytes) were found to be insensitive to ATP4- in this respect. These cells survived the treatment without apparent damage to their alkaline phosphatase activities, osteogenic potentials, and osteoclast induction capacities. The elimination of the endogenous cells of hemopoietic origin from bone tissue or cell populations derived therefrom offers the possibility to study the properties and functions of osteogenic or chondrogenic cells without interference by the presence of cells of hemopoietic origin. It also allows the study of interactions between osteogenic cells and selected cell populations of hemopoietic origin in coculture experiments.
- Subjects :
- Adenosine Triphosphate pharmacology
Animals
Cell Membrane Permeability drug effects
Cell Separation
Fetus cytology
Fibroblasts cytology
Fibroblasts drug effects
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells drug effects
Macrophages cytology
Macrophages drug effects
Male
Membrane Potentials drug effects
Mice
Neutral Red
Osteoclasts cytology
Osteoclasts drug effects
Bone and Bones cytology
Hematopoietic System cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0171-967X
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Calcified tissue international
- Publication Type :
- Academic Journal
- Accession number :
- 7953980
- Full Text :
- https://doi.org/10.1007/BF00297190