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Expression of an Onchocerca volvulus Ov33 homolog in Dirofilaria immitis: potential in immunodiagnosis of heartworm infection.
- Source :
-
Parasite immunology [Parasite Immunol] 1994 Jun; Vol. 16 (6), pp. 297-303. - Publication Year :
- 1994
-
Abstract
- In this study, the expression of an Onchocerca volvulus Ov33 homolog was demonstrated in Dirofilaria immitis. Rabbit antiserum raised against a recombinant fusion protein of O. volvulus, MBP/OvD 5B (Ov33), was found to react with a 31-33 kDa glycoprotein (DiT33) of adult worms of D. immitis. An antibody response to MBP/OvD 5B was observed in dogs, as early as 11 weeks post infection with infective larvae of D. immitis, and in dogs with occult infection. Cats both experimentally and naturally infected with D. immitis also reacted strongly with the recombinant antigen. In contrast, sera from dogs receiving chemically-abbreviated infection or from animals harbouring a variety of other helminths failed to react. These data suggest that antibody responses generated by DiT33 may have potential in immunodiagnosis of heartworm infection in cats and dogs.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Helminth immunology
Cat Diseases diagnosis
Cat Diseases immunology
Cats
Cross Reactions
Dirofilaria immitis genetics
Dirofilariasis immunology
Dog Diseases diagnosis
Dog Diseases immunology
Dogs
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression
Helminth Proteins immunology
Male
Molecular Sequence Data
Onchocerca volvulus genetics
Rabbits
Recombinant Fusion Proteins immunology
Antigens, Helminth immunology
Dirofilaria immitis immunology
Dirofilariasis diagnosis
Onchocerca volvulus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0141-9838
- Volume :
- 16
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Parasite immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7970866
- Full Text :
- https://doi.org/10.1111/j.1365-3024.1994.tb00352.x