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Bony fish neurophysins. Identification of MSEL- and VLDV-neurophysins of the pollack (Pollachius virens).

Authors :
Chauvet J
Lee WJ
Chauvet MT
Acher R
Source :
International journal of peptide and protein research [Int J Pept Protein Res] 1994 Aug; Vol. 44 (2), pp. 130-8.
Publication Year :
1994

Abstract

The two types of neurophysins known in vertebrate species, namely MSEL-neurophysin (vasopressin-like hormone-associated neurophysin) and VLDV-neurophysin (oxytocin-like hormone-associated neurophysin) have been purified from the pollack (Pollachius virens) pituitary through a combination of molecular sieving and high-pressure liquid chromatography (HPLC). Homogeneity has been checked by gel electrophoresis and return in HPLC. The apparent molecular masses measured by SDS-electrophoresis are near 12 kDa, significantly higher than those found for their mammalian homologues (10 kDa). The two types of neurophysins have been recognized through their N-terminal amino acid sequences. The primary structure of MSEL-neurophysin has been partially determined using automated Edman degradation applied on native and reduced-alkylated protein, as well as peptides derived by trypsin or staphylococcal proteinase hydrolyses. Comparison of pollack MSEL-neurophysin with ox, goose and frog counterparts reveals that particular positions in the polypeptide chain are subjected to substitutions and that the numbers of substitutions do not seem closely related to the paleontological times of divergence between the different vertebrate classes.

Details

Language :
English
ISSN :
0367-8377
Volume :
44
Issue :
2
Database :
MEDLINE
Journal :
International journal of peptide and protein research
Publication Type :
Academic Journal
Accession number :
7982756
Full Text :
https://doi.org/10.1111/j.1399-3011.1994.tb00567.x