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Bony fish neurophysins. Identification of MSEL- and VLDV-neurophysins of the pollack (Pollachius virens).
- Source :
-
International journal of peptide and protein research [Int J Pept Protein Res] 1994 Aug; Vol. 44 (2), pp. 130-8. - Publication Year :
- 1994
-
Abstract
- The two types of neurophysins known in vertebrate species, namely MSEL-neurophysin (vasopressin-like hormone-associated neurophysin) and VLDV-neurophysin (oxytocin-like hormone-associated neurophysin) have been purified from the pollack (Pollachius virens) pituitary through a combination of molecular sieving and high-pressure liquid chromatography (HPLC). Homogeneity has been checked by gel electrophoresis and return in HPLC. The apparent molecular masses measured by SDS-electrophoresis are near 12 kDa, significantly higher than those found for their mammalian homologues (10 kDa). The two types of neurophysins have been recognized through their N-terminal amino acid sequences. The primary structure of MSEL-neurophysin has been partially determined using automated Edman degradation applied on native and reduced-alkylated protein, as well as peptides derived by trypsin or staphylococcal proteinase hydrolyses. Comparison of pollack MSEL-neurophysin with ox, goose and frog counterparts reveals that particular positions in the polypeptide chain are subjected to substitutions and that the numbers of substitutions do not seem closely related to the paleontological times of divergence between the different vertebrate classes.
- Subjects :
- Amino Acid Sequence
Animals
Arginine Vasopressin isolation & purification
Arginine Vasopressin metabolism
Molecular Sequence Data
Neurophysins isolation & purification
Neurophysins metabolism
Peptide Fragments chemistry
Protein Precursors isolation & purification
Protein Precursors metabolism
Sequence Analysis
Sequence Homology, Amino Acid
Trypsin metabolism
Arginine Vasopressin chemistry
Fishes
Neurophysins chemistry
Oxytocin
Pituitary Gland chemistry
Protein Precursors chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0367-8377
- Volume :
- 44
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of peptide and protein research
- Publication Type :
- Academic Journal
- Accession number :
- 7982756
- Full Text :
- https://doi.org/10.1111/j.1399-3011.1994.tb00567.x