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Expression of a calcium binding protein pEL98 (mts1) during differentiation of human promyelocytic leukemia HL-60 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1994 Jul 15; Vol. 202 (1), pp. 94-101. - Publication Year :
- 1994
-
Abstract
- The expression of pEL98 (also termed mts1), an S100-related calcium-binding protein, was induced during macrophagic or granulocytic differentiation of human promyelocytic leukemia HL-60 cells in response to phorbol 12-myristate 13-acetate or dimethylsulfoxide, respectively. However, pEL98 expression remained at the low level during granulocytic differentiation of the cells by the treatment with all-trans retinoic acid. The expression of cell motility, but not adhesiveness or phagocytic ability, was found to be coincided with that of pEL98 (mts1), suggesting that pEL98 (mts1) is involved in regulating cell motility. Immunocytochemical analysis demonstrated that pEL98 (mts1) was expressed in human monocytes, macrophages and polymorphonuclear leukocytes.
- Subjects :
- Blotting, Northern
Cell Adhesion drug effects
Cell Differentiation drug effects
Cell Movement drug effects
Dimethyl Sulfoxide pharmacology
Granulocytes cytology
Humans
Kinetics
Leukemia, Promyelocytic, Acute
Leukocytes, Mononuclear cytology
Macrophages cytology
Neutrophils cytology
Phagocytosis
RNA, Messenger biosynthesis
RNA, Messenger isolation & purification
S100 Calcium-Binding Protein A4
Tetradecanoylphorbol Acetate pharmacology
Tretinoin pharmacology
Tumor Cells, Cultured
Calcium-Binding Proteins biosynthesis
Gene Expression
Leukocytes, Mononuclear metabolism
Neutrophils metabolism
S100 Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 202
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 8037774
- Full Text :
- https://doi.org/10.1006/bbrc.1994.1898