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Microwave-antigen retrieval for proliferation analysis (MIB-1) and quanticyt karyometry of bladder washings.
- Source :
-
European journal of morphology [Eur J Morphol] 1994 Mar; Vol. 32 (1), pp. 71-8. - Publication Year :
- 1994
-
Abstract
- Bladder washings can be used for simultaneous karyometry and proliferation analysis using MiB-1 as a proliferation marker. We analyzed 42 problem cases with a discordance between cytologic and karyometric classification (QUANTICYT), in which the karyometric classification was based on a combination of a nuclear shape parameter and DNA (2cDI). Moreover, 25 concordant cases were analyzed: 5 normal samples, 6 low-grade tumors and 7 high-grade tumors. All normal samples and all low-grade tumors had labelling indices below 10%, and all high-grade tumors over 10%. For the discordant low-grade tumors, the QUANTICYT classification correlated better with the MiB-1 labelling than the cytologic diagnosis. There was a clear correlation between 2cDI and MiB-1 labelling index. Slightly elevated MiB-1 labelling indices might have some prognostic value.
- Subjects :
- Antigens, Neoplasm radiation effects
Biomarkers, Tumor radiation effects
Carcinoma, Transitional Cell chemistry
Cell Division
DNA, Neoplasm analysis
Follow-Up Studies
Frozen Sections
Humans
Membrane Proteins radiation effects
Neoplasm Proteins radiation effects
Paraffin Embedding
Protein Denaturation radiation effects
Sensitivity and Specificity
Therapeutic Irrigation
Urinary Bladder chemistry
Urinary Bladder Neoplasms chemistry
Antibodies, Monoclonal immunology
Antigens, Neoplasm analysis
Biomarkers, Tumor analysis
Carcinoma, Transitional Cell pathology
Cell Nucleus ultrastructure
Membrane Proteins analysis
Microwaves
Neoplasm Proteins analysis
Specimen Handling methods
Urinary Bladder pathology
Urinary Bladder Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0924-3860
- Volume :
- 32
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of morphology
- Publication Type :
- Academic Journal
- Accession number :
- 8086270