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Microwave-antigen retrieval for proliferation analysis (MIB-1) and quanticyt karyometry of bladder washings.

Authors :
Van der Poel HG
Boon ME
Source :
European journal of morphology [Eur J Morphol] 1994 Mar; Vol. 32 (1), pp. 71-8.
Publication Year :
1994

Abstract

Bladder washings can be used for simultaneous karyometry and proliferation analysis using MiB-1 as a proliferation marker. We analyzed 42 problem cases with a discordance between cytologic and karyometric classification (QUANTICYT), in which the karyometric classification was based on a combination of a nuclear shape parameter and DNA (2cDI). Moreover, 25 concordant cases were analyzed: 5 normal samples, 6 low-grade tumors and 7 high-grade tumors. All normal samples and all low-grade tumors had labelling indices below 10%, and all high-grade tumors over 10%. For the discordant low-grade tumors, the QUANTICYT classification correlated better with the MiB-1 labelling than the cytologic diagnosis. There was a clear correlation between 2cDI and MiB-1 labelling index. Slightly elevated MiB-1 labelling indices might have some prognostic value.

Details

Language :
English
ISSN :
0924-3860
Volume :
32
Issue :
1
Database :
MEDLINE
Journal :
European journal of morphology
Publication Type :
Academic Journal
Accession number :
8086270