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Dietary fats and properties of endoplasmic reticulum: II. Dietary lipid induced changes in activities of drug metabolizing enzymes in liver and duodenum of rat.
- Source :
-
Lipids [Lipids] 1975 Aug; Vol. 10 (8), pp. 467-72. - Publication Year :
- 1975
-
Abstract
- Rats were fed cholesterol, cacao butter, or olive oil diets to determine the effect of dietary lipids on the rate of drug biotransformation in the liver and duodenum. The cholesterol rich diet maintained the hepatic aryl hydrocarbon hydroxylase activity at the same level as did the standard diet. Rats fed olive oil and cacao butter diets showed lower hepatic aryl hydrocarbon hydrorylase activity. The p-nitroanisole O-demethylase activity was doubled in hepatic microsomes of rats fed the high cholesterol diet when compared to rats fed the standard diet. The hepatic uridine diphosphate glucuronosyltransferase activity showed different patterns depending on the in vitro treatment of the microsomal membranes. If the enzyme activity was assayed from the native, untreated microsomes, an increase in the measurable uridine diphosphate glucuronosyl transferase activity was found in rats having cholesterol rich diet. After the in vitro activation of membrane-bound uridine diphosphate glucuronosyltransferase by trypsin, the increase in measurable activity was 10 fold in the group fed the standard diet, 6 fold in group fed cholesterol, 4 fold in group fed cacao butter, and 3 fold in group fed olive oil. Trypsin digestion of microsomes increased the measurable uridine diphosphate glucuronosyltransferase activity less in rats fed diets rich in neutral fats than those fed the standard diet. In the duodenal mucosa, lipid diets decreased the activities of drug hydroxylation and glucuronidation.
- Subjects :
- Animals
Cholesterol, Dietary
Duodenum drug effects
Endoplasmic Reticulum drug effects
Intestinal Mucosa drug effects
Male
Microsomes drug effects
Microsomes, Liver drug effects
Oils pharmacology
Rats
Trypsin
Aryl Hydrocarbon Hydroxylases metabolism
Dietary Fats
Duodenum enzymology
Endoplasmic Reticulum enzymology
Glucuronosyltransferase metabolism
Hexosyltransferases metabolism
Intestinal Mucosa enzymology
Lipids pharmacology
Microsomes enzymology
Microsomes, Liver enzymology
Nitroanisole O-Demethylase metabolism
Oxidoreductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0024-4201
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Lipids
- Publication Type :
- Academic Journal
- Accession number :
- 808676
- Full Text :
- https://doi.org/10.1007/BF02532430