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Characterization of T-cell receptor gamma delta T cells appearing at the early phase of murine Listeria monocytogenes infection.
- Source :
-
Immunology [Immunology] 1993 Jan; Vol. 78 (1), pp. 22-7. - Publication Year :
- 1993
-
Abstract
- It has been shown that T-cell receptor (TcR) gamma delta + CD4- CD8- T cells increase in number and have an important role in early protection in murine Listeria monocytogenes infection. In this report, to characterize further the phenotype of the gamma delta T cells in listeriosis, we analysed V region gene usage and in vitro antigen recognition of the TcR gamma delta T cells in the peritoneal cavity of mice at the early phase after i.p. infection with a sublethal dose of L. monocytogenes. The gamma delta T cells predominantly expressed V delta 6 which has been reported to be expressed by TcR gamma delta-bearing foetal thymocyte hybridomas specific to mycobacterial and self heat-shock protein (hsp) 60. These early appearing CD3+ CD4- CD8- T cells in Listeria-infected mice, which were reported to be TcR gamma delta T cells, increased in proportion and in size by in vitro stimulation with recombinant hsp 60 from Mycobacterium bovis and purified protein derivative from M. tuberculosis but not by stimulation with heat-killed L. monocytogenes. A 65,000 MW molecule was detected in the lysate of viable L. monocytogenes but not in the lysate of heat-killed L. monocytogenes by a monoclonal antibody (mAb) raised against mycobacterial hsp 60. These results suggest that the V delta 6-bearing peripheral gamma delta T cells are activated by recognizing listerial hsp 60 expressed by viable L. monocytogenes. The hsp 60-reactive V delta 6-bearing T cells may have an important role in protection against L. monocytogenes and other parasites that express hsp 60 at high level.
- Subjects :
- Animals
Blotting, Western
Chaperonin 60
Female
Genes immunology
Heat-Shock Proteins immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta analysis
Receptors, Antigen, T-Cell, gamma-delta genetics
Listeriosis immunology
Receptors, Antigen, T-Cell, gamma-delta analysis
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 78
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 8094708