The 5' untranslated region of the human gamma-glutamyl transferase mRNA contains a tissue-specific active translational enhancer.

Authors :: Diederich M
Wellman M
Visvikis A
Puga A
Siest G
Source :: FEBS letters [FEBS Lett] 1993 Oct 11; Vol. 332 (1-2), pp. 88-92.
Publication Year :: 1993
Abstract: We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell lines shows that this 5'UTR acts as a tissue-specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.

Subjects :: Base Sequence
Cloning, Molecular
Humans
Luciferases genetics
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Messenger chemistry
Tumor Cells, Cultured
beta-Galactosidase genetics
Enhancer Elements, Genetic
Protein Biosynthesis
RNA, Messenger genetics
gamma-Glutamyltransferase genetics

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