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[Reactivation of Escherichia coli inactivated by ultraviolet light by cell extracts of propionic acid bacteria].

Authors :
Vorob'eva LI
Nikitenko GV
Khodzhaev EIu
Ponomareva GM
Source :
Mikrobiologiia [Mikrobiologiia] 1993 Nov-Dec; Vol. 62 (6), pp. 1135-43.
Publication Year :
1993

Abstract

For the first time reactivation of cell extract of three strains of Propionibacterium shermanii in UV inactivated not filament-forming strain Escherichia colli AB 1157 is shown. Reactivation was demonstrated in preincubated and postincubated test-culture and increased as survival of E. coli decreased in a range 1.8-0.006%. The factor (factors) of defense is dialysable, thermolabile and is present as in a fraction of nucleoproteins and nucleic acids so in a fraction of soluble proteins. The extracts were inactivated by incubation with proteinase K and trypsin, partly decreased activity by incubation with alpha-amylase and selected nuclease but not with lipase. Polypeptide nature of reactivating factor is supposed.

Details

Language :
Russian
ISSN :
0026-3656
Volume :
62
Issue :
6
Database :
MEDLINE
Journal :
Mikrobiologiia
Publication Type :
Academic Journal
Accession number :
8114646