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Detection by reversed-phase HPLC of trace amounts of the alpha 113His globin chain in a sheep homozygous for a quadruple alpha-globin gene chromosome.
- Source :
-
The International journal of biochemistry [Int J Biochem] 1993 Dec; Vol. 25 (12), pp. 1939-41. - Publication Year :
- 1993
-
Abstract
- 1. In sheep alpha alpha 113His and alpha D alpha alpha 113His globin gene haplotypes, the percentage gene efficiencies, from the 5' to the 3' end, are about 32-18 and 30-14-6, respectively. 2. We previously found that in a alpha alpha alpha alpha homozygote there may be a 1-2% alpha 113His chains, which however were difficult to resolve from alpha 113Leu chains by CMC chromatography. 3. We report here the experimental conditions which allowed a neat resolution of the 1% alpha 113His chain peak by RP-HPLC. 4. This finding strongly suggests the occurrence of the alpha D alpha alpha alpha 113His haplotype where the percentage gene efficiencies are 30-(14-5)-1, supporting the existence of a gradient of decreasing expression in multiple alpha-globin gene haplotypes.
Details
- Language :
- English
- ISSN :
- 0020-711X
- Volume :
- 25
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The International journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8138032
- Full Text :
- https://doi.org/10.1016/0020-711x(88)90328-x