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Removal of biological stains from aqueous solution using a flow-through decontamination procedure.
- Source :
-
Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 1994 Jan; Vol. 69 (1), pp. 45-54. - Publication Year :
- 1994
-
Abstract
- Chromatography columns filled with Amberlite XAD-16 were used to decontaminate, using a continuous flow-through procedure, aqueous solutions of the following biological stains: acridine orange, alcian blue 8GX, alizarin red S, azure A, azure B, brilliant blue G, brilliant blue R, Congo red, cresyl violet acetate, crystal violet, eosin B, eosin Y, erythrosin B, ethidium bromide, Giemsa stain, Janus green B, methylene blue, neutral red, nigrosin, orcein, propidium iodide, rose Bengal, safranine O, toluidine blue O, and trypan blue. Adsorption was most efficient for stains of lower molecular weight (< 600). Adsorption of stain increased as the flow rate decreased; column diameter had little effect on adsorption. Adsorption of stain was greatest when finely ground resin was used, but if the resin particles were too small, column clogging occurred. Limited grinding of the resin gave increased adsorption while retaining good flow characteristics. Amberlite XAD-16 saturated with methylene blue was regenerated to its initial adsorption capacity by passing methanol through the column. The technique described provides an economical, rapid means of removing stains from aqueous solution.
- Subjects :
- Animals
Chromatography, Ion Exchange
Coloring Agents isolation & purification
Coloring Agents toxicity
In Vitro Techniques
Ion Exchange Resins
Methylene Blue chemistry
Mutagenicity Tests
Particle Size
Polymers
Rats
Resins, Plant
Solutions
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Coloring Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1052-0295
- Volume :
- 69
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biotechnic & histochemistry : official publication of the Biological Stain Commission
- Publication Type :
- Academic Journal
- Accession number :
- 8148434
- Full Text :
- https://doi.org/10.3109/10520299409106260